2013-06-02 22:08:18.0 [sgrebas] added project description update AltLabel (Enzyme Production by Psychrophilic Bacteria);
D - F
EPPB
The objective of this project is to continue the study of Antarctic bacteria able to produce exoenzymes with high activity at low temperatures and evaluate their potential industrial application. In addition, owing to the ecological relevance of these bacterial activities, we decided to make a broad study of the ... bacterial communities from which these strains were isolated. Screenings of psychrophilic bacteria will be made using selective culture media (in Petri dishes or liquid cultures) that permit us to detect the desired enzymatic activities (proteases, amylases, lipases, etc). Strains showing the desired activity will be studied to determine their psychrophilic character and to optimise the extracellular enzymes production under different culture. Subsequently, purification and characterisation of the enzymes showing the biotechnological potential will be performed. Taxonomic studies will be made by sequencing 16S rARN gene and bacterial communities will be analyzed using denaturing gradient gel electrophoresis (DGGE) and restriction fragments length polymorphism (RFLP). Due to the interest showed by many biotechnological industries in the metabolic capabilities of these extremophilic microorganisms agreements are being established in order to develop studies about the industrial application of the genome sequence and the products of these isolated strains.