@prefix ns0: <https://gcmd.earthdata.nasa.gov/kms#> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .

<https://gcmd.earthdata.nasa.gov/kms/concept/9c3c6e76-c7e3-4ba4-8d8e-c6a673fba052>
  ns0:altLabel [ ] ;
  skos:changeNote """2013-06-02 22:08:18.0 [sgrebas] added project description 
update AltLabel (Enzyme Production by Psychrophilic Bacteria); 
""" ;
  skos:broader <https://gcmd.earthdata.nasa.gov/kms/concept/24cf4b0e-4464-4edb-8f0c-415e851a6d79> ;
  skos:prefLabel "EPPB"@en ;
  skos:inScheme <https://gcmd.earthdata.nasa.gov/kms/concepts/concept_scheme/projects> ;
  skos:definition """The objective of this project is to continue the study of Antarctic
      bacteria able to produce exoenzymes with high activity at low
      temperatures and evaluate their potential industrial application. In
      addition, owing to the ecological relevance of these bacterial
      activities, we decided to make a broad study of the ... bacterial
      communities from which these strains were isolated. Screenings of
      psychrophilic bacteria will be made using selective culture media (in
      Petri dishes or liquid cultures) that permit us to detect the desired
      enzymatic activities (proteases, amylases, lipases, etc). Strains
      showing the desired activity will be studied to determine their
      psychrophilic character and to optimise the extracellular enzymes
      production under different culture. Subsequently, purification and
      characterisation of the enzymes showing the biotechnological
      potential will be performed. Taxonomic studies will be made by
      sequencing 16S rARN gene and bacterial communities will be analyzed
      using denaturing gradient gel electrophoresis (DGGE) and restriction
      fragments length polymorphism (RFLP). Due to the interest showed by
      many biotechnological industries in the metabolic capabilities of
      these extremophilic microorganisms agreements are being established in
      order to develop studies about the industrial application of the
      genome sequence and the products of these isolated strains."""@en ;
  a skos:Concept .

<https://gcmd.earthdata.nasa.gov/kms/concept/24cf4b0e-4464-4edb-8f0c-415e851a6d79>
  skos:prefLabel "D - F"@en ;
  a skos:Concept ;
  skos:narrower <https://gcmd.earthdata.nasa.gov/kms/concept/9c3c6e76-c7e3-4ba4-8d8e-c6a673fba052> .